SynMiBi.

Research Synopsis

In my PhD, I develop a novel high-​throughput platform (uASPIre) for sequence-​function mapping of gene regulatory elements in Escherichia coli. I combine a recombinase-based DNA recorder with next-​generation sequencing, which enables us to record sequence-​function data of millions of genetic variants with high phenotypic and temporal resolution at low experimental effort. I capitalize on these features to develop an in-depth understanding of key sequence determinants governing translation in bacteria.